flex analysis v.4.0 software Search Results


gsea software v4.0.3  (Broad Institute Inc)
90
Broad Institute Inc gsea software v4.0.3
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Gsea Software V4.0.3, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/gsea+software/pmc08855819-289-5-16
Average 90 stars, based on 1 article reviews
gsea software v4.0.3 - by Bioz Stars, 2026-10
90/100 stars
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image analysis software slimvision v4.0  (CYBERNET SYSTEMS CO LTD)
90
CYBERNET SYSTEMS CO LTD image analysis software slimvision v4.0
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Image Analysis Software Slimvision V4.0, supplied by CYBERNET SYSTEMS CO LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/image+analysis+software+slimvision+v4+0/10__1507_slash_endocrj__ej17___0517-43-3-5
Average 90 stars, based on 1 article reviews
image analysis software slimvision v4.0 - by Bioz Stars, 2026-10
90/100 stars
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bio plex manager software version 6 1 1  (Bio-Rad)
99
Bio-Rad bio plex manager software version 6 1 1
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Bio Plex Manager Software Version 6 1 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/Bio-Plex+Manager+Software/bio_rxiv__2021__03__28__437432-123-16-10
Average 99 stars, based on 1 article reviews
bio plex manager software version 6 1 1 - by Bioz Stars, 2026-10
99/100 stars
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dragonfly software (v. 4.0  (Object Research Systems (ORS) Inc)
90
Object Research Systems (ORS) Inc dragonfly software (v. 4.0
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Dragonfly Software (V. 4.0, supplied by Object Research Systems (ORS) Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/dragonfly+software/pmc08724477-112-5-9
Average 90 stars, based on 1 article reviews
dragonfly software (v. 4.0 - by Bioz Stars, 2026-10
90/100 stars
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msd discovery workbench analysis software v4.0  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC msd discovery workbench analysis software v4.0
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Msd Discovery Workbench Analysis Software V4.0, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/msd+discovery+workbench+software/pmc08104185-84-3-1
Average 90 stars, based on 1 article reviews
msd discovery workbench analysis software v4.0 - by Bioz Stars, 2026-10
90/100 stars
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rstudio software  (RStudio)
90
RStudio rstudio software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Rstudio Software, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/rstudio+software/pmc11368695__mmc1-98-14-14
Average 90 stars, based on 1 article reviews
rstudio software - by Bioz Stars, 2026-10
90/100 stars
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elispot reader v4.0 software  (AID Diagnostika)
90
AID Diagnostika elispot reader v4.0 software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Elispot Reader V4.0 Software, supplied by AID Diagnostika, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/elispot+reader/pmc04002222-83-14-18
Average 90 stars, based on 1 article reviews
elispot reader v4.0 software - by Bioz Stars, 2026-10
90/100 stars
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sigmastat v 3 11 statistical analysis software  (SYSTAT)
96
SYSTAT sigmastat v 3 11 statistical analysis software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Sigmastat V 3 11 Statistical Analysis Software, supplied by SYSTAT, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/SigmaStat+v4%2E0/pmc06523068-91-7-12
Average 96 stars, based on 1 article reviews
sigmastat v 3 11 statistical analysis software - by Bioz Stars, 2026-10
96/100 stars
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version 4 5 0 software  (Bio-Rad)
99
Bio-Rad version 4 5 0 software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Version 4 5 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/Bio-Plex+Manager+Software+Version+4%2E0/pm18323794-161-26-29
Average 99 stars, based on 1 article reviews
version 4 5 0 software - by Bioz Stars, 2026-10
99/100 stars
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imagelab v4 0 analysis software  (Bio-Rad)
99
Bio-Rad imagelab v4 0 analysis software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Imagelab V4 0 Analysis Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/Image+Lab+Software/pmc09495908-66-6-10
Average 99 stars, based on 1 article reviews
imagelab v4 0 analysis software - by Bioz Stars, 2026-10
99/100 stars
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r software  (STATA Corporation)
99
STATA Corporation r software
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
R Software, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/STATA+4%2E0/pmc11892093-38-6-14
Average 99 stars, based on 1 article reviews
r software - by Bioz Stars, 2026-10
99/100 stars
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software r studio v4.0  (RStudio)
90
RStudio software r studio v4.0
CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis <t>(GSEA)</t> of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)
Software R Studio V4.0, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flex+analysis+v%2E4%2E0+software/rstudio+version+1+3+1093/10__1007_slash_s43076___021___00099___7-99-10-8
Average 90 stars, based on 1 article reviews
software r studio v4.0 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis (GSEA) of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)

Journal: JCI Insight

Article Title: Tumor-infiltrating exhausted CD8 + T cells dictate reduced survival in premenopausal estrogen receptor–positive breast cancer

doi: 10.1172/jci.insight.153963

Figure Lengend Snippet: CD8 + T cells from 8 ER + BC and 2 TNBC patient tissues were single-cell index sorted for whole-transcriptome analysis in the context of several cell surface proteins. ( A ) t-Distributed stochastic neighbor embedding (t-SNE) projection of 4 major clusters of CD8 + T cells identified and annotated as exhausted T cells, resident effector memory T cells, effector memory T cells, and central memory T cells. ( B ) Top 10 most differentially expressed genes for each CD8 + T cell cluster. ( C ) t-SNE overlay of CD8 + T cells identified as PD-1 – CD39 – (blue), PD-1 + CD39 – (orange), PD-1 + CD39 + (red), or PD-1 – CD39 + (gray). ( D ) Genes most significantly differentially expressed by PD-1 + CD39 + CD8 + T cells. ( E ) Overlay of cell surface protein expression onto t-SNE cluster projections. Protein expression for PD-1, CD103, CD69, CD39, CD137, and CCR7 acquired from index sort information and shown here as positively (purple) or negatively (yellow) expressed for each cell. Gene Set Enrichment Analysis (GSEA) of PD-1 + CD39 + CD8 + T cell differentially expressed genes as compared with T EX signatures identified from ( F ) lung cancer and ( G ) melanoma publications. Gene rank shown is derived from the current data set. ( n = 10 BC patients; 9 tumors; 2 T + LNs; 3 NCBTs; 7 matched PBMCs.)

Article Snippet: The GSEA was implemented by GSEA software (v4.0.3) with the latest Hallmark Molecular Signatures Database from Broad Institute ( , ).

Techniques: Expressing, Derivative Assay